Fig. 5

Inhibition of papillary thyroid cancer (PTC) cell proliferation, migration, and invasion by miR-613 directly targeting TAGLN2. A rescue experiment was performed to confirm that miR-613 exerts its inhibitory effect by targeting TAGLN2. a Significant re-upregulation of TAGLN2 in PTC cell lines after TAGLN2 overexpression based on western blot analysis. b Reversal of the inhibitory effects of miR-613 on PTC cell growth at 48, 72, and 96 h by TAGLN2 based on MTS assay. c, d Reversal of the inhibitory effects of miR-613 on cell migration by TAGLN2 based on wound healing assays. Representative images showing the migration of three PTC cells in six-well culture plates (c). Quantification of the cell migration distances corresponding to three PTC cells (d). e, f Reversal of the cell invasion inhibitory effects of miR-613 by TAGLN2. Images of cells that migrated to the bottom chamber (e), and calculated number of invasion cells (f). * p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001