Fig. 2

miR-198 decreases the expression of BIRC5. A Bioinformatics analysis of miR-198 and BIRC5 using TargetScanHuman 7.2. The graphic model of the binding site of miR-198 in the 3′-UTR of BIRC5. B qRT-PCR. miR-198 expression was quantified in 7 pairs of fresh RCC and adjacent tissues. C Luciferase assay. A498 and ACHN cells were co-transfected with BIRC5-WT + pRL-TK, mimic + BIRC5-WT + pRL-TK, miR-198 + BIRC5-WT + pRL-TK, and miR-198 + BIRC5-MUT + pRL-TK; The cells were harvested 48 h later for dual-luciferase assay. D Point mutation model of BIRC5 dual-luciferase reporter vector. BIRC5-WT is the luciferase reporter vector with wild-type BIRC5 3′-UTR, and BIRC5-MUT is the luciferase reporter vector with BIRC5 3′-UTR with a mutated miR-198 binding site. E A498 cells were treated with the scrambled mimic and miR-198; After 48 h, qRT-PCR was performed. **P < 0.01; ****P < 0.0001. F A498 cells were treated as described in (E); after 48 h, the cell lysates were subjected to western blotting with survivin and tubulin antibodies. ****P < 0.0001. G ACHN cells were treated with the scrambled mimic and miR-198; after 48 h, the cells underwent western blotting analysis with indicated antibodies. ****P < 0.0001