Fig. 6

CDH4 was determined as the target of miR-612. A qRT-PCR analysis of CDH4 after overexpression of miR-612. B Western blot analysis of CDH4 protein level in PTC cells transfected with FER1L4 siRNA, miR-612 mimics, or negative control. C Expression of CDH4 in PTC cells transfected with FER1L4 vector, miR-612 inhibitor, or negative control. D The binding sites of miR-612 to FER1L4 3′ UTR were mutated (left panel), and then luciferase activity was detected in TPC-1 cells co-transfected with miR-612 mimics and corresponding report vectors as indicated (right panel). E Western blot analysis of CDH4 protein level in PTC cells co-transfected with FER1L4 siRNA and miR-612 inhibitor. F Detection of CDH4 protein level in PTC cells co-transfected with FER1L4 vector and miR-612 mimics. (G) CDH4 expression was efficiently silenced by siRNA. H, I Detection of cellular proliferation in PTC cells transfected with siRNA against CDH4 using EdU incorporation assays, and CCK8 assays. Scale bars = 100 μm. J, K Flow cytometric analysis of cell apoptosis and cell cycle to cells treated with siRNA targeting CDH4. L Relative expression of Bax, Bcl-2, CDK2, and CyclinA2 in CDH4-depleted PTC cells compared with control. Error bars, mean ± SD. * P < 0.05; ***, P < 0.001