Fig. 2
From: Gly-tRF enhances LCSC-like properties and promotes HCC cells migration by targeting NDFIP2
Gly-tRF is involved in the maintenance of LCSC-like properties. A, C Flow cytometric analysis to detect the percentage of representative LCSC surface markers (CD133, CD13, EpCAM, CD44) in HCCLM3 and Huh7 cells stably transfected with Gly-tRF NC-mimic and Gly-tRF mimic. The statistical graph shows result of three independent experiments. B, D Sphere formation assay to reflect the sphere formation ability of those cells with LCSC-like properties in HCCLM3 and Huh7 transfected with Gly-tRF NC-mimic and Gly-tRF mimic. Scale bar = 100 μm. The statistical graph shows the diameter of spheres in three independent experiments. Data are shown as mean ± SD. ns means no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001