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Fig. 6 | Cancer Cell International

Fig. 6

From: Blocking circ-SCMH1 (hsa_circ_0011946) suppresses acquired DDP resistance of oral squamous cell carcinoma (OSCC) cells both in vitro and in vivo by sponging miR-338-3p and regulating LIN28B

Fig. 6

The interactive role of miR-338-3p and LIN28B in DDP resistance of OSCC cells in vitro. SCC-15/DDP and CAL-27/DDP cells were transfected with miR-NC alone, miR-338-3p alone or together with LIN28B-overexpression vector (LIN28B) or pcDNA vector. A Western blotting detected LIN28B protein level after transfection with normalization to GAPDH. B MTT assay identified IC50 of DDP after transfection. C Colony formation assay measured number of colonies after transfection. D FCM analyzed apoptosis rate after transfection. E, F Transwell assays measured numbers of migrated cells and invaded cells after transfection. G, H Western blotting examined relative protein expression of Bax and cleaved-caspase-3 with correction with GAPDH. **P < 0.01, ***P < 0.001 and ****P < 0.0001 from three separate assays

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Cancer Cell International

ISSN: 1475-2867

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