Fig. 1

METTL3 promotes expression of MALAT1 by modulating the m6A modification of MALAT1 in BC cells. A The METTL3 expression in BC tissues detected by Western blot analysis (n = 68). B The expression of METTL3 in BC cells was detected by Western blot analysis and in BC tissues detected by immunohistochemistry. C The MALAT1 expression in BC tissues measured by qRT-PCR (n = 68). D The expression of MALAT1 in BC cells was detected by qRT-PCR. E Western blot detection results of METTL3 expression level after METTL3 silencing. F qRT-PCR results of METTL3 expression after METTL3 silencing. G After silencing METTL3, m6A modification level of MALAT1 was detected in MCF-7 cells by Me-RIP. H The combination of METTL3 and MALAT1 detected by PAR-CLIP. I: Kaplan-Meier survival curves of BC patients (n = 68). *Significant difference (P < 0.05). The above data were expressed as mean ± standard deviation. Paired t-test was used to compare between BC and adjacent tissues samples, and unpaired t-test was used between the other two groups. One-way ANOVA with Tukey’s post-test was used for data comparison among multiple groups. Kaplan-Meier survival method was used to calculate the survival rate. All experiments were repeated three times