Fig. 3

MALAT1 can sponge miR-26b and restrict miR-26b expression in BC cells. A The miR-26b expression in BC tissue was examined by qRT-PCR, n = 68. B The miR-26b expression in BC cells was detected by qRT-PCR. C The expression of MALAT1 detected by qRT-PCR after MALAT1 was silenced in MCF-7 cells. D The expression of miR-26b in MCF-7 cells was detected by qRT-PCR after knockdown of MALAT1 expression. E Binding of MALAT1 to miR-26b was detected by luciferase reporter gene assay. F The interaction of MALAT1 and miR-26b was verified by RIP assay, and the precipitated MALAT1 and miR-26b were detected by qRT-PCR. G The combination of MALAT1 and miR-26b was confirmed by RNA-pull down assay, and MALAT1 was detected by qRT-PCR. *Significant difference (P < 0.05). The above data were all measurement data and expressed as mean ± standard deviation. Paired t-test was used between BC and adjacent groups. One-way ANOVA with Tukey’s post-test was used for data comparison among multiple groups. All experiments were repeated three times