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Fig. 5 | Cancer Cell International

Fig. 5

From: Lnc-STYK1-2 regulates bladder cancer cell proliferation, migration, and invasion by targeting miR-146b-5p expression and AKT/STAT3/NF-kB signaling

Fig. 5

miR-146b-5p suppresses ITGA2 expression in bladder cancer cells by direct binding. A, B Relative expression of miR-146b-5p and ITGA2 in bladder cancer cells transfected with specific mimics targeting miR-146b-5p. The expression of miR-146b-5p (A) and ITGA2 (B) was detected via qRT-PCR. C Decrease in ITGA2 protein levels in bladder cancer cells induced by miR-146b-5p mimics. ITGA2 protein contents were analyzed by Western blotting. D The predicted binding sites between miR-146b-5p and the 3′ UTR region of the ITGA2 gene by bioinformatics. E The direct association of miR-146b-5p with ITGA2 gene 3′ UTR sequences in 293T cells. A dual-luciferase reporter assay was performed to validate the microRNA and gene sequence interaction. NC: negative control; ITGA2: integrin α2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; UTR: untranslated region; WT: wild type; MUT: mutant type; ***P < 0.001; ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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