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Fig. 8 | Cancer Cell International

Fig. 8

From: CircFAM13B promotes the proliferation of hepatocellular carcinoma by sponging miR-212, upregulating E2F5 expression and activating the P53 pathway

Fig. 8

A Western blotting were performed to determine the protein level of E2F5 in HepG2 and Smmc-7721 transfected with Control + miR-NC, E2F5 + miR-NC, Control + miR-212 mimic and E2F5 + miR-212 mimic. B, C CCK-8 assays were performed to determine the cell proliferation of HepG2 and Smmc-7721 transfected with Control + miR-NC, E2F5 + miR-NC, Control + miR-212 mimic and E2F5 + miR-212 mimic. D, E CCK-8 assays were performed to determine the cell proliferation of HepG2 and Smmc-7721 transfected with Control + si-NC, circFAM13B + si-NC, Control + si-E2F5, circFAM13B + si-E2F5. F The p53 luciferase reporter were detected in HepG2 and Smmc-7721 transfected with Control + si-NC, circFAM13B + si-NC, Control + si-E2F5, circFAM13B + si-E2F5. G Western blotting were performed to determine the protein level of PUMA and P21 in HepG2 and Smmc-7721 transfected with Control + si-NC, circFAM13B + si-NC, Control + si-E2F5, circFAM13B + si-E2F5 (***P < 0.001, **P < 0.01, *P < 0.05)

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Cancer Cell International

ISSN: 1475-2867

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