Fig. 4
From: LPCAT1 overexpression promotes the progression of hepatocellular carcinoma
LPCAT1 induced the proliferation of Huh7 and SMMC-7721 cells. The LPCAT1-knockout Huh7 and SMMC-7721 cells were constructed by using the clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (Cas9) system. a, b Validated the transfection efficiency of LPCAT1-knockout group and control group in Huh7 and SMMC-7721 cell lines, respectively. c, d Proliferation assay was conducted to evaluate the effect of LPCAT1-knockout on cellular proliferation. e–j Flow cytometry was used to detect the cycle distribution and apoptosis of LPCAT1-knockout SMMC-7721 cells. ****P < 0.0001; ***P < 0.001; **P < 0.01