Fig. 2

Macrophage polarization-associated lnc-Ma301 is linked to hepatocellular carcinoma (HCC). A qRT-PCR validation of lnc-Ma301 expression in M1/M2 macrophages and HCC tissues (n = 14) and cell lines. B Agarose gel electrophoresis of the 5’ and 3’ RACE amplification products of lnc-Ma301. C Schematic presentation of full-length lnc-Ma301 showing the extended regions identified by RACE (green). The full-length of lnc-Ma301 was 694 base pairs (bp), amplified from the original 255 bp. D Representative images of RNA FISH showing nuclear localization of lnc-Ma301 (green) in HCC cell line HepG2 cells. Nuclei were stained with DAPI (blue). Scale bar indicates 20 μm. Data are presented as mean ± standard error of the mean (SEM). *P < 0.05; ***P < 0.001