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Fig. 6 | Cancer Cell International

Fig. 6

From: Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression

Fig. 6

The direct target of bufalin. A The binding ability of bufalin with Annexin A2 and DRP1 in the total protein sample from U251 cells as verified by DARTS-western blot experiments. P values were determined using one-way ANOVA. The relative expression value for each sample is shown along with the mean ± SD for each group. *p < 0.05, compared with DMSO control (n = 3). B DARTS-western blot experiment was performed to detect the protein expression levels of HSPA8, HSPA9 and TUBb in pure protein samples of U251 cells after coincubation with bufalin (n = 3). C The protein expression levels of HSPA8, HSPA9 and TUBb in U251 cells after bufalin treatment as detected by western blot analysis (n = 3)

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Cancer Cell International

ISSN: 1475-2867

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