Fig. 3

TFF1 expression alters H. pylori-induced transcriptional activation and regulation of NF-κB and STAT3 target genes. a-b Luciferase activity assay using NF-κB-Luc (a) and STAT3-Luc (b). AGS-pcDNA and AGS-TFF1 cells were co-transfected with NF-κB-Luc or STAT3-Luc and β-galactosidase plasmids. After 24 h, cells were infected with H. pylori 7.13 strain. Cells were collected 4 h after infection for NF-κB-Luc and 24 h for STAT3-Luc measurements.  H. pylori 7.13 infection of AGS-pcDNA cells significantly increased the luciferase activity, which was reduced after the reconstitution of TFF1. The bar graphs represent the mean ± SEM of 3 independent experiments. c–f RT-qPCR analysis showing a decrease in mRNA expression of pro-inflammatory target genes (IL-6,VEGF-α, IL-17 and IL-23) in AGS-TFF1 cells relative to AGS-pcDNA cells, following infection with H. pylori 7.13. The bars represent the mean ± SEM of three independent experiments