Fig. 1

Expression of lncRNA CTD-3252C9.4 in pancreatic cancer cells and tissues. A The cluster heat map of all the significantly down-regulated (> 50%) lncRNAs from lncRNA sequencing analysis in Panc-1 spheroid cells compared to Panc-1 parental cells, including CTD-3252C9.4. B and C Relative expression of CTD-3252C9.4 was detected in PCSCs (B) and different pancreatic cancer cells (C) by qRT-PCR. D Relative expression of CTD-3252C9.4 was detected in MIApaca-2 and Panc-1 cells stimulated by ITS, N2, B27, bFGF and EGF (E and F). Relative expression of CTD-3252C9.4 was detected in 20 non-paired (E) or paired (F) pancreatic cancer tissues and the adjacent normal tissues. G Relative expression of CTD-3252C9.4 was detected in pancreatic cancer patients with different T stages, N stages and M stages. H The relationship between overall survival (OS) of pancreatic cancer patients and CTD-3252C9.4 expression. All data are shown as the mean ± SEM. *P < 0.05 and **P < 0.01 by two-tailed Student’s t-test