Fig. 5

LncRNA CTD-3252C9.4 regulates pancreatic cancer cells migration, invasion, proliferation and apoptosis via targeting IFI6. Proliferation of Panc-1 cells transfected with indicated plasmids was detected by CCK-8 assays (A), plate colony assays (B, Scale bar, 1 cm) and EdU as says (C, Scale bar, 200 μm). Apoptosis of Panc-1 cells transfected with indicated plasmids was detected by Annexin V/ propidium iodide (PI) staining and flow cytometry assays (D) and TUNEL assay (E). Relative expression of apoptosis-related proteins was detected in cells above (F). Bcl-2/Bax ratio was determined by comparing the relative expression levels of Bcl-2 and Bax (G). H Detection of mitochondrial membrane potential by JC-1 staining in Panc-1 cells transfected with indicated plasmids. All data are shown as the mean ± SEM. *P < 0.05 and **P < 0.01 by two-tailed Student’s t-test