Fig. 7

IGF2BP3 promoted melanoma cell proliferation and migration in vitro. A RT-qPCR analysis of IGF2BP3 expression of mRNA in 30 paired fresh melanoma tissues (T) and matched adjacent normal tissues (N) quantified after transfection. B–C Effects of IGF2BP3 overexpression and knockdown on cell proliferation by CCK-8 assays in A375 cells. (P-values were calculated using ANOVA test.). D–E Effects of IGF2BP3 overexpression and knockdown on cell proliferation by colony formation assays in A375 cells. F–G Effects of IGF2BP3 overexpression and knockdown on cell proliferation by EdU staining assay in A375 cells. (Scale bar, 100 μm). H–I Effects of IGF2BP3 overexpression and knockdown on cell migration by wound healing assay in A375 cells. (Scale bar, 2.0 mm). (Data are shown as the mean ± SD of three replicates. For statistical comparison between two independent experimental groups (Student’s t-test) and among more than two experimental groups (ANOVA test), appropriated statistical tests were assayed. *P < 0.05, **P < 0.01, ***P < 0.001.)