Fig. 3

miR-1180-3p is reduced in exosomes from plasma of melanoma patients and in melanoma cells. A Exosomal miR-1180-3p is downregulated in melanoma patients. The reads count of miR-1180-3p in small RNA sequencing was detected as described in Methods. Significant difference was evaluated using Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001. B Exosomal miR-1180-3p is decreased in the validation cohort of melanoma patients. Exosomal RNA was extracted from plasma of melanoma patients (n = 28) and healthy individuals (n = 28), and RT-Q-PCR was performed as described in Methods. Data from multiple experiments are expressed as the mean ± SD (n = 3). Significant differences were evaluated using Student’s t-test, *p < 0.05, ** p < 0.01, ***p < 0.001. C ROC curve of miR-1180-3p. The curve was constructed by the expression value for miR-1180-3p. The Area Under the Curve (AUC) with 95% CI was computed and shown for ROC curve. The Wilcoxon-Mann–Whitney test was used to test the null hypothesis that the AUC is equal to .5 (i.e., no predictive power) and the P values for each test were shown. D miR-1180-3p is decreased in melanoma cells. Total RNAs were extracted from melanocyte (PIG1) and melanoma cells (ME4405, A375, SK-Mel-28, SK-Mel-5), and RT-Q-PCR was performed as described in Methods. Data from multiple experiments are expressed as the mean ± SD (n = 3). Significant differences were evaluated using one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001