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Fig. 6 | Cancer Cell International

Fig. 6

From: The role of XBP-1-mediated unfolded protein response in colorectal cancer progression-a regulatory mechanism associated with lncRNA-miRNA-mRNA network

Fig. 6

The regulatory role of XBP-1u in KCNQ1OT1/XIST associated cell proliferation and apoptosis. A HCT116 cells were transfected with XBP-1u knockdown vector (KD-XBP-1 tv1#1/2) and the negative control (KD NC). The expression level of XBP-1 transcript variant 1 (tv1) and XBP-1 tv2. B HCT116 cells were transfected with KD-XBP-1 tv1 or co-transfected with KD-XBP-1 tv1 and KCNQ1OT1/XIST. CCK-8 assay was performed to detected cell proliferation ability. C TUNEL assay was conducted to detect cell apoptosis. D, E western blot assay was performed to measure the protein expression of XBP-1 s, XBP-1u, Bcl-2, cleaved caspase3 and caspase3. **, ***p < 0.01, 0.001 vs. KD NC; #, ##, ###p < 0.05, 0.01, 0.001 vs. KD XBP-1 tv1

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Cancer Cell International

ISSN: 1475-2867

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