Fig. 4

SIN3A was the downstream mRNA of miR-138-5p in CRC cells.   A, B 8 possible mRNAs were predicted through RNA22, miRmap, miRanda, PicTar and TargetScan databases. C qRT-PCR detected expression of the 8 mRNAs in cells after miR-138-5p mimics transfection. D The expression of SIN3A was quantified in cells with transfection of indicated plasmids via qRT-PCR. E SIN3A expression in CRC cell lines and FHC was measured via qRT-PCR. F, G RIP and RNA pull down assay were done for evaluating the relationship of LINC00665, miR-138-5p and SIN3A. H The binding site of SIN3A and miR-138-5p was obtained on ENCORI. I Interaction of SIN3A and miR-138-5p was reflected through luciferase reporter experiment. J SIN3A overexpression efficacy of was tested by qRT-PCR. K SIN3A expression was quantified by applying qRT-PCR in cells with indicated transfection. *P < 0.05, **P < 0.01, n.s no significance