Fig.4

Altered PGM1 expression affects glucose and lipid metabolism under glucose deprivation. a–d Colorimetry assays to measure glycolysis of BGC-823 and MKN-28 cells with knockdown of PGM1 or NC under the NG or LG condition, including lactate acid (a), pyruvic acid (b), ATP (c) production and glucose uptake capability (d). e WB assay to show protein expression levels of cells as in (a–d). f Colorimetry assay to measure FASN activity of cells as in (a–d). Data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. no significant. PGM1: Phosphoglucomutase 1; FASN: Fatty Acid Synthase; CPT1A: Carnitine Palmitoyltransferase 1A; ACC: Acetyl CoA Carboxylase; NC: negative control; NG: normal glucose, 10 mM glucose in medium; LG: low glucose, 2.5 mM glucose in medium; SD: standard deviation