Fig. 2

P5091 induces apoptosis in GBM cells

A SHG-140 and T98G cells were treated with P5091 for 24, 48, and 72 h. Cell viability was determined by CCK-8 assay. B The apoptosis of SHG-140 and T98G cells treated with different concentrations of P5091 for 48 h was measured by flow cytometry, n = 3. Q3 (Annexin V-FITC + PI-) subpopulation was considered early-stage apoptosis and Q2 (Annexin V-FITC + PI+) was late-stage apoptosis or necrosis. Cell proportion comparisons between groups were done in both early- and late-stage subpopulation separately and in total. C, D. SHG-140 and T98G cells were treated with P5091 for 48 h. Changes in apoptotic proteins were observed by western blotting analysis, n = 3. E Immunofluorescence analysis of SHG-140 and T98G, cells were stained with DAPI and antibodies against BCL-2 or CLEAVED-CASPASE 3. Scale bar, 100 μm. Statistics are shown as **P < 0.01, ***P < 0.001 or ****P < 0.0001