Fig. 3
From: An insertion variant of MGMT disrupts a STAT1 binding site and confers susceptibility to glioma
a rs10659396 resides within STAT1 DNA-binding motif. b EMSA with biotin-labeled oligonucleotides containing the rs10659396 ins allele (Probe-ins) or del allele (Probe-del) and nuclear extracts from U251 cells. Lanes 1 and 4 show mobilities of the labeled oligonucleotides without nuclear extracts; lanes 2 and 5 show mobilities of the labeled oligonucleotides with nuclear extracts in the presence of Non-labeled del or Non-labeled ins competitors; lanes 3 and 6 show mobilities of the labeled oligonucleotides with nuclear extracts in the absence of competitor; lanes 7 and 8 show mobilities of the labeled oligonucleotides with nuclear extracts in the presence of STAT1 antibody. c ChIP assays using U251 cells with rs10659396 del/del genotype. Representative gel shows the PCR verification results from input, STAT1 ChIPed DNA, and nonspecific IgG ChIPed DNA. d Knockdown of STAT1 by RNAi diminishes the mRNA levels of MGMT. Columns indicate means; Bars represent SE. ***P < 0.001. e Depletion of STAT1 by RNAi decreases in the protein levels of MGMT