Fig. 4

Integrative analysis of CREB1-upregulated gene network. A Gene distribution analysis of four different gene lists (R circlize package). They are (i) CREB1 commonly upregulated gene list; (ii) RRHO concordantly upregulated gene list; (iii) commonly opposite regulated genes between CREB1 rescue cells vs corresponding KO clones and KO clones vs WT cells; (iv) CREB1 CHIP-seq gene list (genes with p value ≤ 0.001, CREB1 binding ratio ≥ 2 are selected). The stripes of different colors connect the portions uniquely shared by different combinations of the lists (i–iv) (Additional file 5: Table S4). Three genes (red stripe) are present in all 4 lists. Twenty six genes (blue stripe) are uniquely shared by i, ii and iv. B 29 top-ranked CREB1-upregulated targets illustrated by their average log₂ FC (Rescue/KO) and the tumor occurrence number in RRHO concordant gene list. The scale of log₂ FC is displayed by the tomato gradient bar. C, D CREB1 binding sites in CHIP-seq data, gene function annotation based on GO analysis and detailed tumor occurrence of the 29 overlapped genes