Fig. 2

AL355338 promoted NSCLC cell proliferation in vitro. A Representative FISH images showed the sub-cellular location of AL355338 in A549 and PC9 cells (blue, DAPI; red, positive staining). Scale bar, 20 μm. B Expression of AL355338 in cytoplasmic and nuclear fractionations of A549 cells. C The knockdown efficiency of AL355338 in A549 and PC9 cells after being transfected with two siRNAs (si-AL355338#1 and si-AL355338#2). D, F Relative cell cycle distribution was evaluated by flow cytometry assays in A549 and PC9 transfected with AL355338 siRNAs or negative control (si-NC). E, G Cell proliferation was performed by CCK-8 assay over a 3-day period in A549 and PC9 cells transfected with AL355338 siRNAs or si-NC. H Cell colony formation assay was used to assess the ability of cell proliferation in indicated cells. Scale bar, 100 μm. I Immunofluorescence analysis of EdU was performed in AL355338 knockdown cells after 24 h. Scale bar, 50 μm. Data shown are mean ± SD (n = 3) (*P < 0.05, **P < 0.01, ***P < 0.001)