Fig. 2

UBTF promotes melanoma cell proliferation and restrains apoptosis in vitro. a UBTF mRNA level was measured in A375/SK-MEL-28 cells after transfection with UBTF siRNAs. b UBTF mRNA level was detected after UBTF overexpression. c MTT assay revealed melanoma cell activity after transfection with UBTF siRNAs. d MTT assay showed melanoma cell activity after transfection with UBTF overexpression vector. e Cell colonies were measured 11 days after transfection with UBTF siRNAs. f Cell colonies were detected 11 days after transfection with UBTF overexpression vector. g Cell cycle was analyzed by using flow cytometry. The histograms revealed the proportion of G1/G0, S and G2/M phase cells after transfection with UBTF siRNAs. h The histograms showed the percentage of G1/G0, S and G2/M phase cells after transfection with UBTF overexpression vector. i The data represented the percentage of apoptosis after transfection with UBTF siRNAs. j The data revealed the ratios of apoptosis after transfection with UBTF overexpression vector. k Bcl-2/Bax expressions were detected after transfection with UBTF siRNAs. l Bcl-2/Bax expressions were measured after transfection with UBTF overexpression vector. *p < 0.01, n = 3