Fig. 7

UBTF facilitates melanoma cell growth through modulating GIT1-MEK1/2-ERK1/2 pathways. a GIT1 mRNA level was measured in A375/SK-MEL-28 cells after co-transfection with UBTF siRNA-1 and GIT1 overexpression vector. b MTT assay revealed melanoma cell multiplication after co-transfection with UBTF siRNA-1 and GIT1 overexpression vector. c Cell colonies were detected 11 days after co-transfection. d Cell cycle was examined after co-transfection. e Apoptosis was analyzed after co-transfection. f GIT1-MEK1/2-ERK1/2 signalling pathways were measured after co-transfection. g ERK1/2 signalling pathways were detected after co-treatment with UBTF overexpression vector and U0126. *p < 0.01, as compared with NC-siRNA group; ^#p < 0.01, as compared with UBTF siRNA-1 + vector group; n = 3