Fig. 2

Overexpression of miR-140-3p inhibited the proliferation, invasion, and migration of GC cells. miR-140-3p lentivirus overexpression vector was transfected into AGS cells with low expression. miR-140-3p inhibitor was transfected into MKN45 cells with high expression. A The expression of miR-140-3p in GC cells was detected using RT-qPCR. The proliferation of cells was detected by CCK-8 assay (B) and clone formation assay (C). D, E The invasion and migration of cells were detected by Transwell assays. The experiment was repeated3 times independently. Data were presented as mean ± standard deviation. Comparisons among panels in A, C, D, E were performed using the t-test. Comparison of data in B was performed using two-way ANOVA, followed by Tukey's multiple comparisons test. **p < 0.01. LV-oe-miR: The lentiviral overexpression vector of miR-140-3p; LV-oe-NC: negative control of lentiviral overexpression vector; inhibitor: miR-140-3p inhibitor