Fig. 3

Comparison of the wild-type and N-MYC-P44L interactome. A Summary of proteins enriched in wild-type and P44L mutant N-MYC complexes. The x axis displays the enrichment (log2 fold-change) of proteins in wild-type HA-N-MYC-expressing cells compared to control cells, while the y axis displays the enrichment in HA-N-MYC-P44L expressing cells, both calculated from the mean of biological duplicates. Previously characterized N-MYC interactors are depicted in green, novel interactors that were tested via immunoprecipitation in blue. The size of dots correlates with the number of identified razor and unique peptides of the corresponding protein. Dots located close to the solid diagonal line represent proteins equally enriched in both complexes, while dots beyond the dotted lines were more strongly enriched either in the wild-type or the mutant complexes. B Immunoblots of HEK293 cells transiently transfected with expression vectors for HA-N-MYC and candidates for which binding to N-MYC is potentially affected by the P44L mutation. White asterisks represent the specific bands of N-MYC interactor candidates in the input and FLAG co-IP. Vinculin input served as a loading control