Fig. 5

 A The immunohistochemistry (IHC) of brain tissue sections of mice. The black arrows represent astrocyte, the blue arrows indicate brain tissue, and the red arrows indicate the tumor. Figure d is an enlarged portion of the red frame in Figure c. B The immunofluorescence (IF) of brain tissue sections of mice. The nuclei were stained by Hoechst. GFAP and CD31 were specific markers of astrocytes and endothelial cells, respectively. The normal brains in figure A and B were obtained by the brain tissues of health mice without injection of tumor cells, the brain metastases were observed on the sixth day after injection of tumor cells