Fig. 6

YAP1 is regulated by miR-509-3p and ASAP1-IT1. A The potential target of miR-509-3p is predicted by TargetScan. B qRT-PCR analysis of YAP1 expression in NSCLC tissues and adjacent tissues, *p < 0.01 compared with adjacent tissues. C qRT-PCR analysis of YAP1 in A549 spheres, *p < 0.01 compared with parental group. D Luciferase activity of pmirGLO-YAP1-3′UTR-WT (wild type) or pmirGLO-YAP1-3′UTR-Mut (mutant) was modulated by miR-509-3p and ASAP1-IT1 in A549 cells, *p < 0.01 compared with Mock + pcDNA, φp < 0.01 compared with miR-509-3p + pcDNA, Фp < 0.01 compared with miR-509-3p + ASAP1-IT1. E YAP1 mRNA levels in A549 cells analyzed byqRT-PCR, *p < 0.01 compared with Mock + pcDNA, φp < 0.01 compared with miR-509-3p + pcDNA, Фp < 0.01 compared with miR-509-3p + ASAP1-IT1. F Western blot analysis on YAP1 protein in A549 cells