Fig. 7

Overexpression of YAP1 reversed miR-509-3p-mediated cancer cell stemness, cell growth, and apoptosis of A549-derived stem cells. A Number of tumorA549 spheres after transfection with Mock, pcDNA, miR-509-3p, and YAP1, *p < 0.01 compared with Mock + pcDNA, φp < 0.01 compared with miR-509-3p + pcDNA. B qRT-PCR analysis on stemness-related genes (SOX2, CD44, and CD133)48 h after transfection in A549-derived stem cells, *p < 0.01 compared with Mock + pcDNA, φp < 0.01 compared with miR-509-3p + pcDNA. C Annexin V-FITC/PI staining of A549-derived stem cells after transfection with Mock, pcDNA, miR-509-3p, and YAP1, *p < 0.01 compared with Mock + pcDNA, φp < 0.01 compared with miR-509-3p + pcDNA. D qRT-PCR analysis on apoptosis-associated genes (Bax, Bcl-2, and caspase-3) in A549-derived stem cells, *p < 0.01 compared with Mock + pcDNA, φp < 0.01 compared with miR-509-3p + pcDNA. E Colony formation assay on cell growth of A549-derived stem cells after 15 days culture, *p < 0.01 compared with Mock + pcDNA, φp < 0.01 compared with miR-509-3p + pcDNA. F qRT-PCR analysis of growth-associated f genes (Cyclin A1, Cyclin B1, and PCNA) in stem cells of A549 cells, *p < 0.01 compared with Mock + pcDNA, φp < 0.01 compared with miR-509-3p + pcDNA