Fig. 5

Knockout of METTL3 and WTAP impairs proliferation and survival of CRC cells. A METTL3 KO suppresses cell proliferation of HCT-116 cells, as determined by CCK-8 assay. B METTL3 KO inhibits colony formation of HCT-116 cells. Left, the representative picture of cell colonies; right, the statistical result. C and D Cell proliferation and colony formation assay in METTL3 KO DLD-1 cells. E KO of WTAP suppressed cell proliferation of HCT-116 cells. Cell proliferation was determined by CCK-8 assay. F WTAP KO inhibits colony formation of HCT-116 cells. Left, the representative picture of cell colonies; right, the statistical result. G and H Cell proliferation and colony formation assay in WTAP KO DLD-1 cells. Error bars represent SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.005