Fig. 6

IGF2BP2 serves as a reader for m6A modified UCA1. A The workflow of in vivo S1m-tagging RNA pulldown assay. B Detection of the YTHDF1, YTHDF2 and YTHDF3 by western blot, after in vivo RNA pulldown. C Detection of the IGF2BP1, IGF2BP2 and IGF2BP3 by western blot, after in vivo RNA pulldown. D The interaction between UCA1 and IGF2BP2 was confirmed by RIP assay. Error bars represent SD, n = 3, ***P < 0.005. E Mutation on m6A motif of UCA1 impairs the interaction of IGF2BP2 with UCA1. Left, the schematic of the A to C mutation on UCA1 m6A motif; right, the representative western blot showing that the mutant UCA1revealed little interaction with IGF2BP2 and UCA1, in contrast to WT UCA1