Fig. 7

The recognition by IGF2BP2 enhances UCA1 RNA stability. A silencing of IGF2BP2 suppressed IGF2BP2 RNA expression, as determined RNA expression, as determined by western blot. B IGF2BP2 siRNA2 suppresses UCA1 RNA level, as determined by qRT-PCR. C IGF2BP2 siRNA2 decreases UCA1 RNA stability. HCT-116 cells were transfected with IGF2BP2 siRNA2 or NC siRNA. Two days after transfection, the cells were treated with actinomycin D (2 μg/ml) for indicated times. UCA1 RNA stability was determined by qRT-PCR. D Analysis of TCGA database suggests that IGF2BP2 mRNA level is higher in CRC samples than in normal tissue. E Analysis of GEO database (GSE39582) indicates the differential expression of IGF2BP2 in CRC tissue and normal tissue. F qRT-PCR analysis of IGF2BP2 expression in our cohort of 46 matched CRC tumor tissues and adjacent normal tissues; Error bars represent SD, n = 3 (B&C), *P < 0.05, **P < 0.01, ***P < 0.005