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Fig. 2 | Cancer Cell International

Fig. 2

From: Circ-RNF121 regulates tumor progression and glucose metabolism by miR-1224-5p/FOXM1 axis in colorectal cancer

Fig. 2

Circ-RNF121 silencing repressed CRC progression and cell glycolysis. A The silencing efficiency of si-circ-RNF121 was detected by qRT-PCR in HCT-116 and SW480 cells. B, C MTT assay and cell colony formation assay were performed to investigate the impact of circ-RNF121 silencing on cell proliferation in HCT-116 and SW480 cells. D The impact of circ-RNF121 knockdown on the apoptosis of HCT-116 and SW480 cells was determined by flow cytometry assay. E, G Transwell migration assay and wound-healing assay were carried out to analyze the influence of circ-RNF121 repression on the migration of HCT-116 and SW480 cells. F The effect of circ-RNF121 silencing on cell invasion was disclosed by transwell invasion assay in HCT-116 and SW480 cells. H, I Glucose assay kit and lactate assay kit were employed to determine the effects of circ-RNF121 knockdown on glucose consumption and lactate production, respectively, in HCT-116 and SW480 cells. J ADP/ATP ratio assay kit was performed to determine the ratio of ATP and ADP. **P  < 0.01, ***P  < 0.001 and ****P  < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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