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Fig. 2 | Cancer Cell International

Fig. 2

From: Lactate induces aberration in the miR-30a–DBF4 axis to promote the development of gastric cancer and weakens the sensitivity to 5-Fu

Fig. 2

DBF4 promotes the proliferation of GC cells. A, F The qRT-PCR was conducted to measure DBF4 expression in MGC-803 (left) and AGS (right) cells transfected with si-DBF4 (A) or DBF4 overexpression vector (F). B, G DBF4 protein levels were detected by western blots in MGC-803 (above) and AGS (below) cells transfected with si-DBF4 (B) or DBF4 overexpression vector (G). C, H Cell growth analysis by CCK-8 assay in MGC-803 and AGS cells after DBF4 silencing (C) or DBF4 overexpression (H). D, I Colony formation assays were used to measure the proliferation of MGC-803 (left) and AGS (right) cells transfected with si-DBF4 (D) or DBF4 overexpression vector (I). Western blotting assays were used to analyze the expression of cyclin A and PCNA in MGC-803 and AGS cells transfected with si-DBF4 (E) or DBF4 overexpression vector (J). Data are displayed as the mean ± SD of three independent experiments. ***P< 0.001, ****P< 0.0001

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Cancer Cell International

ISSN: 1475-2867

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