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Fig. 5 | Cancer Cell International

Fig. 5

From: CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis

Fig. 5

VASP acted as the target gene of miR-1179 and miR-1229. A The complementary sequences between VASP and miR-1179 or miR-1229 were shown. B The interaction between VASP and miR-1179/miR-1229 was verified using dual-luciferase reporter assay. C and D Following A172 and U251 cells were transfected with miR-NC, miR-1179 or miR-1229, the protein level of VASP was measured by western blot assay. E and F A172 and U251 cells were transfected with sh-NC, sh-circRFX3#1, sh-circRFX3#1 + anti-NC, sh-circRFX3#1 + anti-miR-1179 or sh-circRFX3#1 + anti-miR-1229, and then western blot assay was conducted for VASP protein level. *P < 0.05, **P < 0.01

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