Fig. 6
From: YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells
The genes regulated by VPS25 in glioma. A Heat map representing the DEGs regulated by VPS25 KD. B KEGG pathway analysis showing the most enriched pathways of downregulated genes after VPS25 KD. C Western blotting was used to determine the levels of downstream target proteins in VPS25-silenced U87MG and U251 cells. D Western blotting was used to determine the levels of p-JAK1 and p-STAT1 in VPS25 KD tumor tissue in vivo. E RTCA xCELLigence assay showed that transfection of a VPS25-encoding plasmid promoted the proliferation of U251 cells. F Transfection of the VPS25-encoding plasmid promotes the colony formation of U251 cells. G Western blotting was used to determine the levels of p-JAK1 and p-STAT1 in VPS25 overexpressed U251 cells. β-Actin was used as a loading control. NC: negative control. siVPS25: VPS25 gene silencer; VPS25: VPS25 overexpression plasmid. *P < 0.05, **P < 0.01, ***P < 0.001