Fig. 7
From: YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells
m6A modification is associated with VPS25 expression in glioma cells. A m6A RIP-qPCR analysis showed that m6A is highly enriched within the VPS25 transcript in U87MG and U251 cells. B The expression of VPS25 was detected in U87MG and U251 cells with METTL3 KD. C The expression of VPS25 was detected in U87MG, and U251 cells with METTL14 KD. D The expression of VPS25 was detected in U87MG and U251 cells with YTHDC1 KD. E Western blot was used to detect the VPS25 expression in METTL3, METTL14, or YTHDC1 KD cells. F U251 glioma cells were used to evaluate proliferation after being transfected with siVPS25 and siYTHDC1. *P < 0.05, **P < 0.01, ***P < 0.001. ns: no significant, NC: negative control. siMETTL3: METTL3 gene silencer; siMETTL14: METTL14 gene silencer; siYTHDC1: YTHDC1 gene silencer; siVPS25: VPS25 gene silencer