Fig. 6

MF treatment induces the release of cell-free DNA into the media supernatant. Graphs show number of wild type (A) and mutant (B) copies of cfDNA per μl of cell-free media obtained 72 h after incubation with vehicle, 5, 10, 20, or 40 µM MF. C Representative one-dimensional plot of mutant GNAQ/GNA11 or wild type cfDNA extracted from conditioned media after treatment for 72 h with the depicted concentrations of MF. Channel compatible with FAM dye shows droplets with mutant target in blue. Wild type target is shown in green using a HEX label. Threshold (pink line) set in between positive (mutant or wild type) and no DNA target (black) droplets