Fig. 7From: Anticancer effects of mifepristone on human uveal melanoma cellsThe effect of MF in UM cells is independent from the classical nuclear progesterone receptor. SybrGreen-based Real Time PCR quantified the gene expression profiles of PR, PAQR8, PGRMC1, PGRMC2, and NR3C1. β-Actin was used as a reference gene. mRNAs were amplified from either untreated cells or cells treated with 20 µM MF. The mRNA from MCF-7 cells was used as a positive control for the expression of the classical PR. No template control and no reverse transcriptase control were added in each assay. Individual runs were performed in triplicatesBack to article page