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Fig. 4 | Cancer Cell International

Fig. 4

From: Promotion of gastric tumor initiating cells in a 3D collagen gel culture model via YBX1/SPP1/NF-κB signaling

Fig. 4

SPP1 activated NF-κB signals to promote gastric cancer progression. A Western blotting of NF-κB in SGC-7901/BGC-823 (Dish), 3D cultured SGC-7901/BGC-823 (SCR), 3D cultured YBX1 KO SGC-7901/BGC-823 (Y-KO1, Y-KO2) and 3D cultured SPP1 KO SGC-7901/BGC-823 cells (S-KO1, S-KO2). B SGC-7901/BGC-823 cells were cultured in 3D collagen gels and treated with PBS or JSH-23 (50 nM). The colony growth was examined. The scale bar is 50 μm. C SGC-7901/BGC-823 cells in B were collected and the colony formation capability was examined in dish. D SGC-7901/BGC-823 cells in B were collected and treated with 5-FU (1 µg/ml) for 48 h. The cell apoptosis was examined using Annexin/PI staining. BE SGC-7901/BGC-823 cells in B were collected and treated with PTX (2 µg/ml) for 48 h. The cell apoptosis was examined using Annexin/PI staining

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Cancer Cell International

ISSN: 1475-2867

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