Fig. 5

CircTP63 functions as a sponge of miR-217. A Schematic of the wild-type (WT) or mutant-type (MUT) circTP63 luciferase reporter vectors was constructed (left). The luciferase activities of the circTP63-WT or circTP63-MUT luciferase reporter vector in NOZ cells transfected with miR-217 mimic or miR-NC (right). B The relative expression of miR-217 was detected after NOZ or SGC-996 cells were transfected with si-NC, si-circTP63-1 or si-circTP63-2 by qRT-PCR analysis. C The relative expression of miR-217 was detected in GBC tissues and adjacent normal tissues by qRT-PCR analysis (left). Higher circTP63 expression negatively associated with lower miR-217 expression (right). D The relative expression of miR-217 was detected in three GBC cell lines (NOZ, SGC-996 and GBC-SD) and the human intrahepatic biliary epithelial cell line HIBEC by qRT-PCR analysis. E The bio-miR-217 or NC group complex was pulled down by incubating the cell lysate with streptavidin-coated magnetic beads and the circTP63 was detected by qRT-PCR. F The cell proliferation ability was analyzed by CCK8 assays after cells were transfected with si-NC, miR-217 inhibitor or si-circTP63-1  +  miR-217 inhibitor in NOZ cells. G The cell invasion ability was analyzed by transwell assays after cells were transfected with si-NC, miR-217 inhibitor or si-circTP63-1  +  miR-217 inhibitor in NOZ cells. H, I The cell cycle progression was analyzed by flow cytometry after cells were transfected with si-NC, miR-217 inhibitor or si-circTP63-1  +  miR-217 inhibitor in NOZ cells. Data are shown as mean  ±  SD, *P  <  0.05, **P  <  0.01