Fig. 6

EZH2 is a downstream target of miR-217 and is indirectly regulated by circTP63. A The relative expression of EZH2 was detected in GBC tissues and adjacent normal tissues by qRT-PCR analysis. B Schematic of the wild-type (WT) or mutant-type (MUT) EZH2 luciferase reporter vectors was constructed. C, D The luciferase activities of the EZH2-WT or EZH2-MUT luciferase reporter vector in NOZ and SGC-996 cells transfected with miR-217 mimic or miR-NC. E, F The relative mRNA expression of EZH2 was detected in after NOZ or SGC-996 cells were transfected with si-NC, si-circTP63-1, si-circTP63-2, miR-217 inhibitor and si-circTP63-1  +  miR-217 inhibitor by qRT-PCR analysis. G, H The relative protein expression of EZH2 was detected in after NOZ or SGC-996 cells were transfected with si-NC, si-circTP63-1, si-circTP63-2, or si-NC, miR-217 inhibitor and si-circTP63-1  +  miR-217 inhibitor by western blot analysis, respectively. Data are shown as mean  ±  SD, *P  < 0.05