Fig. 4

GALNT7 is a direct target of mir-30b-5p. A Identification of 2 commonly changed targeted mRNAs of mir-30b-5p from the five publicly profile datasets (TargetScan, miRDB, miRmap, PITA and miRanda) and TCGA datasets. Different color areas represented different datasets. The cross areas meant the commonly changed mRNAs of mir-30b-5p. The height of the column represents the predicted number of genes. B Validation of GALNT7 target genes by real-time PCR. C Western Blot confirms downregulation of GALNT7 protein levels in the mir-30b-5p mimic group. D Quantification of C. E Mir-30b-5p mimic prominently reduced luciferase activity in GALNT7-wild, not in GALNT7-mut. Data are presented as the mean ± SD (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)