Fig. 6

YY1 activates EMI2 and promotes the proliferation of CCA cells. A PROMO and ChIPBase databases were used to identify YY1 and STAT4 as the transcription factors of EMI2, and YY1 was found to be differentially expressed in cholangiocarcinoma; B The OS curve of YY1 and the correlation between YY1 and EMI2 based on TCGA database; C, D The protein levels of YY1 and EMI2 after siYY1 and oeYY1 detected using western blotting; E A diagram showing the binding sites of YY1 and EMI2 using JASAR database; F The effect of YY1 on the fluorescence of GV272-EMI2-WT and GV272-EMI2-MUT was tested using dual-luciferase reporter gene assay; G, H Cell cloning was used to detect the proliferation in siYY1, siEMI2, siYY1 + siEMI2, oeYY1, oeEEMI2, and oeYY1 + oeEMI2 groups. I CCK-8 assay was used to detect the proliferation of cells in each group (*P < 0.05; **P < 0.01; ***P < 0.001)