Fig. 1

DMAMCL induced NB cell apoptosis in vitro. Four NB cell lines (NGP, AS, KCNR, BE2) and NIH3T3 cells were treated with different DMAMCL concentrations. A Cell confluence was monitored dynamically using Incucyte ZOOM for 72 h, ns (not significant): control vs DMAMCL treatment, *p < 0.05; **p < 0.01; ***p < 0.001. B Cell survival was detected using a CCK-8 assay at 72 h. C Colony formation assays were performed in the four NB cells and NIH3T3. Data were presented as mean ± SD. D Cell cycle was analyzed by detecting the percentage of PI-staining cells at each stage using flow cytometry at 48 h treatment of DMAMCL. E Cell apoptosis was detected using Annexin V-PE/7-AAD flow cytometry at 48 h. Data represent the mean ± SD of three independent experiments. F Cleaved PARP expression was detected using western blotting after DMAMCL treatment for 0, 8, 16 and 24 h