Fig. 4

Overexpression of miR-206 and miR-613 inhibits cell growth and the cell cycle in vitro and in vivo. A, B CCK-8 assay of cell viability in PrCa cell lines transfected with miR-206 and miR-613 mimics or inhibitors at 24, 48, 72 and 96 h. C Representative micrographs and D relative quantification of crystal violet-stained cell colonies analyzed by a colony formation assay. E, F Flow cytometric analysis of DU145 cell lines. Cells were harvested at 72 h after transfection with the indicated miRNA and stained with propidium iodide. The percentage of cells in each cell cycle phase is shown in the inset of each panel. G miR-206 and miR-613-overexpressing DU145 cell xenografts in nude mice (n = 6) at the experimental endpoint; tumors were dissected and photographed as shown. Tumor growth curves in mice inoculated with the indicated cells on the indicated days. H Each tumor formed was weighed. I–K HnRNPK mRNA and miR-206 and miR-613 expression in tumors was detected by qRT–PCR analysis. L, M PC-3 and DU145 cells were treated with or without miR-206, miR-613 mimics or the miR-206, miR-613 inhibitor for 72 h, respectively. The expression levels of the indicated proteins were analyzed by immunoblotting. The results were plotted as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001