Fig. 6

Blockade of the MOR and Src/PI3K/AKT/mTOR pathways inhibit the cell cycle progression and antiapoptotic effects of morphine. a Representative images of flow cytometry analyses used to detect the progression of the cell cycle induced by drugs in H460 cells. b Quantitative analysis of the cell cycle indicated in (a). c Representative images from AV/PI flow cytometry used to detect the inhibition of apoptosis induced by drugs in H460 cells. d Quantitative analysis of apoptosis indicated in (c). e The levels of Bcl-2, Bax, cleaved Caspase-3, cleaved Caspase-9, and cleaved PARP were analyzed using Western blotting. f Quantitative analysis of Bcl-2/Bax protein expression. g Quantitative analysis of the level of the cleaved Caspase-3 protein. h Quantitative analysis of the level of the cleaved Caspase-9 protein. i Quantitative analysis of the level of the cleaved PARP protein. In all experiments, cells were treated with the drugs for 48 h. The concentrations of morphine and MNTX were 0.1 μg/μL, the concentration of PP1 was 5 μM, and the concentration of deguelin was 1 μM. The control groups were treated with NS and DMSO at the same volumes. All data are shown as the means ± SD, *p < 0.05, **p < 0.01 compared with the control group; ^#p < 0.05, ^##p < 0.01 compared with the morphine group