Fig. 6

MiR-29b repressed NSCLC cell processes and angiogenic ability by binding to HIF1A. A The overexpression efficiency of HIF1A was detected by western blot in A549 and H1299 cells. B The effects between miR-29b and HIF1A on HIF1A protein expression under hypoxia were revealed by western blot in A549 and H1299 cells. C, D Transwell assay was performed to demonstrate the influences between miR-29b and HIF1A overexpression on the migration and invasion of A549 and H1299 cells under hypoxia. E, F The influences between miR-29b mimic and HIF1A overexpression on cell proliferation under hypoxia were unveiled by cell colony formation and MTT assays in A549 and H1299 cells. G The effects between miR-29b and HIF1A on PCNA protein expression under hypoxia were revealed by western blot in A549 and H1299 cells. H The effects between miR-29b and HIF1A on tube formation expression under hypoxia were revealed by tube formation assay in A549 and H1299 cells. *P < 0.05